Bioscreen

For "typical" bioscreen runs: use 150 µl total volume around 1/100  to 1/300 dilution from YPD overnight


 * If using synthetic media (e.g., SC-URA) in the bioscreen, you must add 0.2% NP40, otherwise the cells will clump together and fall out of suspension.

Bioscreen Instructions:                  Set # of wells (usually 100 or 200 – use the arrow to set up to 200)
 * Check fluid levels, fill if necessary (50% ethylene glycol in drawer)
 * Check and set parameters every run

                 Set temperature (30C normal)

                 Set length of run (usually 24hrs for YPD)

                 Measurement interval (30mins normal)

 Settings – Shaking
 * Make sure shaking is ON

 Continuous – High
 * Make sure the lid is placed back on over the bioscreen plates