Generate rho0 yeast


 * Grow ON of yeast in SD 2% glucose + 25 µg/ml EtBr
 * Culture a second ON from 1st ON, in SD 2% glucose + 25 µg/ml EtBr
 * Plate for single colonies on YPD plate (let grow 2-3 days 30C)
 * every clone should be rho0 (colonies should be small/slow)
 * verify no growth on YPG and slower growth on YPD
 * bioscreen in YPD media and look for slower Dt vs WT and no diauxic shift
 * back cross with WT strain to compliment YPG growth deficiency
 * verify by DAPI staining using log phase cells
 * grow cells to mid log (OD ~0.4-0.6) in SD 2% (if possible, avoid YPD, since it gives lot of background?)
 * Add DAPI ( 1 mg/ml stock) to the final concentration of 2.5 µg/m
 * Grow the cells for 30 min w/DAPI
 * spin down cells, wash with 1X PBS,resuspend the cells in PBS,
 * perform microscopy (use WT and known rho0 cells as controls)
 * perform microscopy (use WT and known rho0 cells as controls)