Yeast media

YEP
 Y east e xtract is what is sounds like, the extract of yeast thus it contains many nutrients yeast would normally use to grow

Bacto P eptone - is an enzymatic digest of animal protein (is a source of amino acids and of nitrogen, at least partially via amino acids e.g., glutamate, glutamine, proline). supposedly contains some trace glucose according to lab lore

We use Bacto peptone and Bacto yeast extract we have issues with other brands, so only use these to be safe...

For 1 L YEP (after autoclave and then adding sugar, it will be YEPD aka YPD, or YPG, etc)

10g Bacto Yeast Extract (i.e., final conc 1%)

20g Bacto Peptone (i.e., final conc 2%)

20g Agar (if making plates) (i.e., final conc 2%)

add water ~960ml if using glucose

autoclave in 2L flask

after cooling somewhat (~won't burn you)

add 40ml of 50% glucose (final concentration 2%) or 60ml 50% glycerol for 3%gly

FOR SD droupout media (1L) (e.g. -LEU, -etc)

1.7g YNB

5.0 g ammonium sulfate

X g of amino acid dropout mix (e.g., CSM-LEU) - the amount will be indicated on the bottle

water to 1L (minus the volume of sugar solution you will add after autoclaving)

autoclave, add sugar after autoclaving

if you are making SD media with agar, make it to 500ml (2X) and after autoclaving mix with 500ml 4%(2X) agar

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Synthetic media terminology and composition
SD is s ynthetic d efined media = YNB, Nitrogen, Carbon source, and CSM amino acids

SC s ynthetic c omplete media = YNB, Nitrogen, Carbon source, and SC amino acids.

YNB is yeast nitrogen base. Contains salts, vitamins and trace elements. Many companies YNB are lacking ammonium sulfate. Verify by the bottle or with the company whether product actually contains nitrogen. (details below)

DOB is dropout base = YNB + Nitrogen + Carbon source

Nitrogen = ammonium sulfate

Carbon source= usually the sugar glucose, or another carbon source, galactose, glycerol, ethanol, etc.

all of these can be +/- agar (sometimes they'll add an 'A' at the end of the name if containing agar. e.g., DOBA)

info from www.sunrisescience.com: and more info from MP; SC and CSM composition below are identical for sunrise and MP biosciences /

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