Protocols

YEAST
Yeast transformation stationary (LKL 2014 protocol)

Yeast Transformation High Efficiency old school - use this one

Yeast Transformation High Effeicieny newer

Yeast Transformation High Effeciency

Yeast integration overview

Yeast media

Mating / Sporulation / Tetrads

Genomic DNA isolation 'smash & grab'

Colony PCR

Yeast spotting assay

Yeast suppressor screen protocol (on this wiki)

setup for RLS
RLS dissection

CLS

Bioscreen

replica plating (link to Mark's wiki)

generate rho0 yeast

mtDNA loss

Other
PCR template

Western blot

BCA assay

Gateway cloning

Sequencing at UW

Drug stocks

Qiagen Kits
Miniprep

PCR cleanup

Gel Extraction

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PCR toolbox info from 2004 paper "A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes"

More protocols
find more protocols here-> - http://getyourscienceon.wikia.com/wiki/Protocols 

and some here -> http://getyourscienceon.wikia.com/wiki/GDMC_protocols